
ProductCategory:Pre-maderecombinantAAV;AAV-hSynapsin-DIO-GFP;AAV-Synapsin-DIO-GFP;AAV9-hSyn-DIO-GFP;AAV9-Syn-DIO-GFP
Description:
AAV9-hSyn-DIO-GFPisapre-maderAAVinSEROtype9whichwillCredependentlyover-expressGFPdrivenunderhumansynapsinpromoterasillustratedasthecartoonbelow.
GFPisagreenmonomerwhichmaturesextremelybright,makingitpossIBLetoseeresultsverysoonafteractivatingtranscription.Itishighlyphotostableandresistanttophotobleaching.IntheDIOscenario,thetransgeneofinterestisinsertedinreverseorientationrelativetothe5'promoterandisflankedbyoppositelyorientedloxPandlox2272sites. IntheabsenceofCreexpression,thetransgenewillnotbeproduced.InthepresenceofCreexpression,thetransgenewillbe"Flip-exchanged"orFLEXed,leADIngtotheexpressionofthetransgene.ThisisduetoapermanentCre-mediatedrecombination/inversionoftheflankedtransgene.ThisarrangementiscalledDIO(double-floxedinverseORF),Cre-ON,Flex-rev(reverse),Flex-ON/FlexON,orDIO-AAV/AAV-DIO(double-floxedinverseORFinAAV).
Titer:>1x1013VG/mL
Size:>30µL(StdPack)
StorageBuffer:SterilePBS
InfectionProtocol:
VectorMap: